Anti-rat IgG Alpha Donor Beads, 1 mg | PerkinElmer

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Anti-rat IgG Alpha Donor Beads, 1 mg

Alpha Donor beads conjugated to anti-rat IgG antibody. This bead can be used to capture rat IgG antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
AS110D
1 mg
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AS110M
5 mg
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AS110R
25 mg
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详情 信息

Alpha Donor beads coated with anti-rat IgG antibody. These beads can be used to capture rat IgG antibodies and rat Fc-fusion proteins, and can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex Acceptor beads to create no-wash assays for:

  • Analyte detection assays
  • Antibody detection assays
  • Antibody binding assays
  • Protein-protein interaction assays
  • Protein detection assays
  • Enzymatic assays

 

幸运8平台彩票In a typical Alpha assay, 1 mg of Donor beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume. Bead concentration can be adjusted for optimal performance.

 

规格

抗体偶联物 Anti-rat IgG
自动化兼容 Yes
珠型或核心珠型 Alpha Donor
检测方法 Alpha
实验类型 In vitro
产品品牌名称 Alpha
运输条件 蓝冰
产品尺寸 1 mg
资源,活动及更多信息
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产品手册

Handle Large Biomolecular Interactions With Ease

幸运8平台彩票The interactions and bindingof proteins are implicated in a large number of biological processes. The needfor an efficient, highly sensitive assay to study large protein interactions is increasingly important. Alpha Technology is a highly flexible, homogeneous, no-wash assay ideal for the measurement of protein interactions and complexes as large as 200 nm in size

PDF 798 KB

指南

Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored, and applications such as phage display, ELISA, and EMSA (electrophoretic mobility shift assay) have been adapted to Alpha.

PDF 380 KB
ELISA to AlphaLISA Immunoassay Conversion Guide

幸运8平台彩票This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

PDF 1 MB
User's Guide To Alpha Assays Protein:Protein Interactions

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for Amplified Luminescent Proximity Homogeneous Assay. The assay does not require any washing steps. Binding of proteins or other binding partners captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent signal.

PDF 3 MB